omentin  (Shanghai Korain Biotech Co Ltd)

Journal: Arthritis Research & Therapy

Article Title: Omentin-1 reduced synovial M1 macrophages through SIRT6 signaling pathway and alleviated knee osteoarthritis response in mice

doi: 10.1186/s13075-025-03680-y

Figure Lengend Snippet: Omentin-1 alleviated pain in ACLT induced SIRT6 −/− OA mice. A Timeline of animal experiments. We performed ACLT surgery to construct wild-type and SIRT6 −/− osteoarthritis (OA) mouse model, and injected 50µL Omentin-1 (300ng/joint/mouse) into the joint cavity of OA mice once a week for 5 consecutive weeks. Behavioral assessment was also performed through pain detection (PWTL) and gait analysis. Knee joint tissue was collected from mice at week 6, with a portion fixed in 4% paraformaldehyde for pathological staining and the remaining portion stored at −80℃ for Western blot analysis. The experimental groups were divided into three groups: Sham group, ACLT group, and Omentin-1 group, with 8 mice in each group. B Pain in OA mice was assessed before surgery and at 1, 2, 3, 4, 5, and 6 weeks after ACLT surgery. (C-E) At the 6th week after surgery, we conducted gait analysis and recorded the swing time, stride length, and paw area of the right hind limb. * p < 0.05, ** p < 0.01, *** p < 0.001

Article Snippet: 24 h after ACLT surgery, two types of mice were injected with Omentin-1 (300ng/50μL, HY-P701099, MedChemExpress) [ ] twice a week into the joint cavity for six consecutive weeks.

Techniques: Construct, Injection, Staining, Western Blot

Journal: Arthritis Research & Therapy

Article Title: Omentin-1 reduced synovial M1 macrophages through SIRT6 signaling pathway and alleviated knee osteoarthritis response in mice

doi: 10.1186/s13075-025-03680-y

Figure Lengend Snippet: Omentin-1 upregulated SIRT6 expression in OA mice. Inject 50µL of Omentin-1 into the joint cavity for 5 weeks, then collect samples at week 6. A First, we assessed SIRT6 expression in the synovial tissues of WT mice using a western blot. B Next, we measured Omentin-1 expression in the synovial tissues of WT and SIRT6 −/− OA mice after Omentin-1 injection. *** p < 0.001; ns. indicated no significance

Article Snippet: 24 h after ACLT surgery, two types of mice were injected with Omentin-1 (300ng/50μL, HY-P701099, MedChemExpress) [ ] twice a week into the joint cavity for six consecutive weeks.

Techniques: Expressing, Western Blot, Injection

Journal: Arthritis Research & Therapy

Article Title: Omentin-1 reduced synovial M1 macrophages through SIRT6 signaling pathway and alleviated knee osteoarthritis response in mice

doi: 10.1186/s13075-025-03680-y

Figure Lengend Snippet: Omentin-1 modulated SIRT6 to facilitate cartilage repair in OA mice model. One week following ACLT surgery, Omentin-1 was administered into the knee joint cavity of OA mice for five weeks. In the 6th week, the mice were subjected to anesthesia and subsequently euthanized, after which knee joint tissues were harvested. Representative images of pathological staining techniques include: ( A ) Hematoxylin and Eosin (H&E) staining, ( B ) Safranin O and Fast Green staining, and ( C ) Toluidine Blue staining. ( D ) Markin score. ( E ) The expression of proteins associated with cartilage repair, Collagen II and Aggrecan in cartilage tissues, was further validated through western blot analysis. * p < 0.05, ** p < 0.01, *** p < 0.001

Article Snippet: 24 h after ACLT surgery, two types of mice were injected with Omentin-1 (300ng/50μL, HY-P701099, MedChemExpress) [ ] twice a week into the joint cavity for six consecutive weeks.

Techniques: Staining, Expressing, Western Blot

Journal: Arthritis Research & Therapy

Article Title: Omentin-1 reduced synovial M1 macrophages through SIRT6 signaling pathway and alleviated knee osteoarthritis response in mice

doi: 10.1186/s13075-025-03680-y

Figure Lengend Snippet: Omentin-1 modulated SIRT6 to suppress the M1 macrophage phenotype while promoting the M2 macrophage phenotype in the synovial tissue of mice with arthritis. A HE staining was utilized to assess the extent of inflammatory infiltration within the synovial tissue. B Arthritis score. Representative immunohistochemical images depicting iNOS ( C ) and CD206 ( D ) in synovial tissue. * p < 0.05, ** p < 0.01, *** p < 0.001

Article Snippet: 24 h after ACLT surgery, two types of mice were injected with Omentin-1 (300ng/50μL, HY-P701099, MedChemExpress) [ ] twice a week into the joint cavity for six consecutive weeks.

Techniques: Staining, Immunohistochemical staining

Journal: Arthritis Research & Therapy

Article Title: Omentin-1 reduced synovial M1 macrophages through SIRT6 signaling pathway and alleviated knee osteoarthritis response in mice

doi: 10.1186/s13075-025-03680-y

Figure Lengend Snippet: Omentin-1 promoted the transformation of Raw264.7 cells into the M2 phenotype while inhibiting the conversion to the M1 phenotype. Raw264.7 cells were induced to differentiate into M1 macrophages by LPS and IFN-γ, while they were induced to differentiate into M2 macrophages by IL-4 and IL-13. After 24 h, Omentin-1 was added to the cell culture medium, and the incubation was continued for an additional 24 h. A Western blot was used to determine the relative expression of Omentin-1 protein in Raw264.7 cells. B - C Representative immunofluorescence images were used to observe the polarization of Raw264.7 cells into M1 macrophages (F4/80 + iNOS + ) and M2 macrophages (F4/80 + CD206 + ). F4/80 (green), iNOS and CD206 (red), nucleus (blue). D - E RT-qPCR was employed to assess the expression levels of pro-inflammatory cytokines secreted by M1 macrophages, IL-1β, IL-6, and TNF-α, as well as anti-inflammatory cytokines secreted by M2 macrophages, IL-10, IL-4 and TGF-β, in Raw264.7 cells. * p < 0.05, ** p < 0.01, *** p < 0.001

Article Snippet: 24 h after ACLT surgery, two types of mice were injected with Omentin-1 (300ng/50μL, HY-P701099, MedChemExpress) [ ] twice a week into the joint cavity for six consecutive weeks.

Techniques: Transformation Assay, Cell Culture, Incubation, Western Blot, Expressing, Immunofluorescence, Quantitative RT-PCR

Journal: Arthritis Research & Therapy

Article Title: Omentin-1 reduced synovial M1 macrophages through SIRT6 signaling pathway and alleviated knee osteoarthritis response in mice

doi: 10.1186/s13075-025-03680-y

Figure Lengend Snippet: Omentin-1 upregulated the expression of SIRT6 in Raw264.7 cells. A Representative western blot images of SIRT6 protein. After stimulation with inflammatory factors, Raw264.7 cells were incubated with Omentin-1 for 24 h, followed by the collection of cell pellets and extraction of total protein for western blot analysis. B Following lentiviral transfection of Raw264.7 cells with SIRT6-OE and SIRT6-KD constructs, western blot was employed to assess the expression levels of SIRT6. C - D After transfection with SIRT6-KD and SIRT6-OE lentiviruses, Raw264.7 cells were stimulated with LPS/IFN-γ or IL-4/IL-13 for 24 h, and then incubated with Omentin-1 for 24 h, western blot was applied for the detection of Omentin-1 and SIRT6 proteins. * p < 0.05, ** p < 0.01, *** p < 0.001; ns. indicated no significance

Article Snippet: 24 h after ACLT surgery, two types of mice were injected with Omentin-1 (300ng/50μL, HY-P701099, MedChemExpress) [ ] twice a week into the joint cavity for six consecutive weeks.

Techniques: Expressing, Western Blot, Incubation, Extraction, Transfection, Construct

Journal: Arthritis Research & Therapy

Article Title: Omentin-1 reduced synovial M1 macrophages through SIRT6 signaling pathway and alleviated knee osteoarthritis response in mice

doi: 10.1186/s13075-025-03680-y

Figure Lengend Snippet: Omentin-1 regulated SIRT6 to inhibit M1 polarization while promoting M2 polarization in Raw264.7 cells. LPS/IFN-γ and IL-4/IL-13 were used to induce Raw264.7 cells, as well as SIRT6-KD and SIRT6-OE Raw264.7 cells, for 24 h. After 24 h incubation with Omentin-1, immunofluorescence analysis was conducted to assess the distribution of ( A ) F4/80 + iNOS + macrophages and ( B ) F4/80 + CD206 + macrophages. ( C - D ) Additionally, RT-qPCR was employed to measure the mRNA expression levels of IL-1β, IL-6, TNF-α, IL-10, TGF-β, and IL-4 in cells. * p < 0.05, ** p < 0.01, *** p < 0.001; ns. indicated no significance

Article Snippet: 24 h after ACLT surgery, two types of mice were injected with Omentin-1 (300ng/50μL, HY-P701099, MedChemExpress) [ ] twice a week into the joint cavity for six consecutive weeks.

Techniques: Incubation, Immunofluorescence, Quantitative RT-PCR, Expressing